Journal: eLife

Article Title: Inter-organ Wingless/Ror/Akt signaling regulates nutrient-dependent hyperarborization of somatosensory neurons

doi: 10.7554/eLife.79461

Figure Lengend Snippet: ( A ) A schematic diagram outlining the observation of C4da neurons in larvae reared on the different diets. We collected wandering 3rd instar larvae and imaged C4da neurons as scheduled throughout our dietary or genetic interventions. This is because the larval development took longer on LYD or LYD +nutrients compared to HYD. ( B–E ) Images of ddaC neurons in larvae reared on LYD ( B ) or LYD +essential amino acids (LYD +EAA; C ). Two-dimensional (2D) plot ( D ) and densities of branch terminals (E, one-way ANOVA and Tukey’s HSD test, n=6). The 95% confidence elipse of LYD +EAA almost totally overlaps with that of LYD and remains largely separare from that of HYD ( D ). ( F–I ) Images of ddaC neurons in larvae reared on LYD +1 x amino acids, which is the same concentration as in the holidic medium (LYD +1 x AAs; F ), or LYD +3 x AAs ( G ). 2D plot ( H ) and densities of branch terminals (I, Steel test, n=8). Terminal density on LYD +1 x AAs was significantly lower than on LYD, whereas that on LYD +3 x AAs was higher ( I ). In contrast to these opposoite effects, both the elipse of LYD +1 x AAs and that of LYD +3 x AAs partly overlap with the LYD elipse and both are located apart from that of HYD ( H ). These effects of supplementing amino acids to LYD make sharp contrast to that of supplementing VMCO or VMC . ( J–L ) Images of ddaC neurons in larvae reared on LYD +peptone ( J ). 2D plot ( K ) and densities of branch terminals (L, Steel test, n=8). The elipse of LYD +peptone almost totally overlaps with that of LYD ( K ). The complete compositions of the diets can be found in . Boxplots in (E, I, and L) are depicted as in . **p<0.01. Scale bar, 100 μm.

Article Snippet: For the supplementation with essential amino acid solution, we used 50 x MEM EAA solution (Wako 132–15641).

Techniques: Concentration Assay

Journal: eLife

Article Title: Inter-organ Wingless/Ror/Akt signaling regulates nutrient-dependent hyperarborization of somatosensory neurons

doi: 10.7554/eLife.79461

Figure Lengend Snippet:

Article Snippet: For the supplementation with essential amino acid solution, we used 50 x MEM EAA solution (Wako 132–15641).

Techniques: Purification, Fluorsave, Software

Journal: eLife

Article Title: Cells use molecular working memory to navigate in changing chemoattractant fields

doi: 10.7554/eLife.76825

Figure Lengend Snippet:

Article Snippet: Chemical compound, drug , MEM Amino Acids Solution (50 x) , PAN Biotech , Cat. # P08 32,100 , .

Techniques: Recombinant, Modification, Software